Journal: PLoS ONE
Article Title: Enhancement of Cell Membrane Invaginations, Vesiculation and Uptake of Macromolecules by Protonation of the Cell Surface
doi: 10.1371/journal.pone.0035204
Figure Lengend Snippet: HaCaT cells were harvested, suspended in HBSS and exposed to pH 5.25 in the presence of 1µM 43kDa dextran-FITC. Following treatment cells were analyzed by FACS calibrated with MESF microspheres. (A) The cells were exposed to pH 5.25 for total periods of 5, 10 15 and 20 minutes where dextran-FITC was added only for the last 5 minutes. The exposure was terminated by dilution in a large volume of cold DMEM. Results of the FACS analysis are given in terms of the number of dextran molecules per cell (mean±SD). The difference between the four groups is insignificant (P>0.05 in One-way ANOVA, n = 12).
Article Snippet: Quantum FITC MESF kit (Bangs Labs, USA) was used in the quantification of FITC fluorescence intensity in units of Molecules of Equivalent Soluble Fluorochrome (MESF).
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